Molecular and functional characterization of odorant-binding protein genes in Holotrichia oblita Faldermann
文献类型： 外文期刊
第一作者： Wang, Chao-Qun
作者： Wang, Chao-Qun;Li, Jin-Qiao;Li, Er-Tao;Nyamwasa, Innocent;Li, Ke-Bin;Zhang, Shuai;Yin, Jiao;Wang, Chao-Qun;Li, Jin-Qiao;Peng, Yu;Wei, Zhao-Jun
作者机构：
关键词： Holotrichia oblita; Odorant binding proteins; RNA interference
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）
ISSN： 0141-8130
年卷期： 2019 年 136 卷
页码：
收录情况： SCI
摘要： Holotrichia oblita is one of the nastiest pests in China. In present research, four full-length cDNA encoding of HoblOBP genes were cloned and sequenced from H. oblita. The mRNA of HoblOBPs were predominantly expressed in antenna. The recombinant HoblOBPs proteins were obtained for fluorescence binding assays. Four of HoblOBPs could mediate the response of H. oblita to organic fertilizers-derived attractants, including Hob1OBP5 binding to skatole; HoblOBP8 binding to p-cresol, indole and skatole; HoblOBP9 binding to indole and 4-allylanisole; and HoblOBP24 binding to p-cresol, indole and 4-ethylphenol. Further, RNA interference demonstrated that transcripts of HoblOBP.5, 8, 9, and 24 decreased in a time-dependent manner after dsRNA-injection. Knockdown of HoblOBP5, 8, 9, and 24 by injection of dsRNA successfully interfered with behavioral responses towards the target compounds in beetles. Our results showed that HoblOBP5, HoblOBP8, HoblOBP9 and HoblOBP24 are essential in mediating the approach behavior of H. oblita. (C) 2019 Elsevier B.V. All rights reserved.
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