Protective immunity induced by DNA vaccine encoding viral membrane protein against SGIV infection in grouper

文献类型： 外文期刊

第一作者： Yu, Nai-Tong

作者： Yu, Nai-Tong;Zheng, Xiao-bao;Liu, Zhi-Xin;Yu, Nai-Tong

作者机构：

关键词： Ranavirus; Singapore grouper iridovirus; Grouper Epinephelus coioides; Membrane protein; DNA vaccine; Protective immunity

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：4.581； 五年影响因子：4.851 ）

ISSN： 1050-4648

年卷期： 2019 年 92 卷

页码：

收录情况： SCI

摘要： Singapore grouper iridovirus (SGIV) is the main grouper-infecting virus in southern China that causes serious economic losses. However, there is no effective way to control this viral disease. In this study, SGIV ORP19R (SGIV-19R) encoding a viral membrane protein was constructed into pcDNA3.1-HA and then was used to evaluate the immune protective effects in grouper Epinephelus coioides. Subcellular localization showed that SGIV-19R distributed in the cytoplasm and co-localization analysis indicated the protein partially co-localized with the endoplasmic reticulum (ER). RT-PCR and Western blot analyses confirmed the expression of the vaccine plasmids in grouper muscle tissues. Moreover, the transcription levels of tumor necrosis factor alpha (TNF-alpha), interleulcin-1 beta (IL-1 beta), myxovirus resistance 1 (Mx1) and immunoglobulin M (IgM) genes were significantly up-regulated in the spleen, liver and kidney of vaccinated groupers. SGIV challenge experiments showed the relative percent survival (RPS) was significantly enhanced in fish with 49.9% at the DNA dose of 45 mu g pcDNA3.1-19R, while 75.0% RPS when using 90 mu g pcDNA3.1-19R. Meanwhile, vaccination with pcDNA3.1-19R significantly reduced the virus replication, evidenced by a low viral load in the spleen of survivals groupers after SGIV challenge. These results imply that pcDNA3.1-19R could induce protective immunity in grouper, and might be a potential vaccine candidate for controlling SGIV disease.

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