EGFR promotes the proliferation of quail follicular granulosa cells through the MAPK/extracellular signal-regulated kinase (ERK) signaling pathway

文献类型： 外文期刊

第一作者： Wu, Yan

作者： Wu, Yan;Xiao, Hongwei;Pi, Jinsong;Zhang, Hao;Pan, Ailuan;Pu, Yuejin;Liang, Zhenhua;Shen, Jie;Du, Jinping;Wu, Yan

作者机构：

关键词： Quail; follicular granulosa cells; follicle development; EGFR; MAPK; ERK

期刊名称：CELL CYCLE （ 影响因子：4.534； 五年影响因子：4.492 ）

ISSN： 1538-4101

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收录情况： SCI

摘要： Follicles develop into preovulatory follicles during folliculogenesis and the majority of small yellow follicles become atretic and gets reabsorbed. In this study, based the RNA-seq results of duck ovary, epidermal growth factor receptor (EGFR) was selected as a candidate gene in follicular development and the role was explored. The results demonstrated that EGFR-P8 was the quail EGFR core promoter. It had an E2F4 binding site within EGFR core promoter. E2F4 overexpression significantly increased EGFR expression in quail granulosa cells (GCs). However, the effect was abolished when the GCs were treated with corynoxeine, an inhibitor of the mitogen-activated protein kinase/extracellular regulated protein kinase (MAPK/ERK) signaling pathway. Moreover, luciferase reporter assay and chromatin immunoprecipitation experiments showed that E2F4 upregulated the expression of EGFR expression, which increased E2 and P4 production. In addition, EGFR regulated GCs proliferation and affected follicular development. Taken together, our findings suggested that EGFR, which was regulated by E2F4, enhanced the expression of MAPK/ERK pathway components and follicular development. These results provided an important basis for an improved understanding of the MAPK/ERK pathway and new insight into the development of quail follicles.

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