FgPex3, a Peroxisome Biogenesis Factor, Is Involved in Regulating Vegetative Growth, Conidiation, Sexual Development, and Virulence in Fusarium graminearum

文献类型： 外文期刊

第一作者： Kong, Xiangjiu

作者： Kong, Xiangjiu;Zhang, Hao;Wang, Xiaoliang;Xu, Jin;Xu, Jingsheng;Chen, Wanquan;Feng, Jie;van Der Lee, Theo;Waalwijk, Cees;van Diepeningen, Anne;Brankovics, Balazs;Kong, Xiangjiu

作者机构：

关键词： Fusarium graminearum; peroxisome; FgPex3; fungal growth; virulence

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Peroxisomes are involved in a wide range of important cellular functions. Here, the role of the peroxisomal membrane protein PEX3 in the plant-pathogen and mycotoxin producer Fusarium graminearum was studied using knock-out and complemented strains. To fluorescently label peroxisomes' punctate structures, GFP and RFP fusions with the PTS1 and PTS2 localization signal were transformed into the wild type PH-1 and Delta FgPex3 knock-out strains. The GFP and RFP transformants in the Delta FgPex3 background showed a diffuse fluorescence pattern across the cytoplasm suggesting the absence of mature peroxisomes. The Delta FgPex3 strain showed a minor, non-significant reduction in growth on various sugar carbon sources. In contrast, deletion of FgPex3 affected fatty acid beta-oxidation in F graminearum and significantly reduced the utilization of fatty acids. Furthermore, the Delta FgPex3 mutant was sensitive to osmotic stressors as well as to cell wall-damaging agents. Reactive oxygen species (ROS) levels in the mutant had increased significantly, which may be linked to the reduced longevity of cultured strains. The mutant also showed reduced production of conidiospores, while sexual reproduction was completely impaired. The pathogenicity of Delta FgPex3, especially during the process of systemic infection, was strongly reduced on both tomato and on wheat, while to production of deoxynivalenol (DON), an important factor for virulence, appeared to be unaffected.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序