Fine mapping of a quantitative trait locus qHD3-1, controlling the heading date, to a 29.5-kb DNA fragment in rice

文献类型： 外文期刊

第一作者： Wang, W. Y.

作者： Wang, W. Y.;Liu, X.;Ding, H. F.;Liu, W.;Yao, F. Y.;Jiang, M. S.;Li, G. X.;Zhu, C. X.

作者机构：

关键词： Oryza sativa; heading date; single segment substitution line; physical mapping; sequence information

期刊名称：RUSSIAN JOURNAL OF PLANT PHYSIOLOGY （ 影响因子：1.481； 五年影响因子：1.608 ）

ISSN： 1021-4437

年卷期： 2011 年 58 卷 3 期

页码：

收录情况： SCI

摘要： In our previous studies, a single segment substitution line (SSSL) W23-03-8-9-1 with substituted interval of PSM301-PSM306-PSM305-PSM304-RM3894-RM3372-RM569-RM231-RM545 on chromosome 3 has been found to comprise a gene for extremely early heading date. To map this gene, the SSSL W23-03-8-9-1 was crossed with the recipient Huajingxian (HJX74) to develop an F2 segregating population. The distribution of early and late heading plants in this population fitted a segregation ratio of 3: 1, indicating that early heading was controlled by a dominant gene. Using a random sample of 520 individuals from the F2 segregation population, the qHD3-1 locus was mapped between two SSR markers, RM3894 and RM3372, with genetic distances of 1.2 and 1.1 cM, respectively. For fine mapping of qHD3-1, a large F-2: 3 segregating population was developed, with 6000 individuals from the F-2 plants heterozygous in the RM3894 and RM3372 regions. The analysis of recombinants in the qHD3-1 region put the gene locus into an interval of 29.5 kb flanked by the left marker 3HD8 and the right marker 3HD9. Sequence analysis of this fragment predicted eight open reading frames. One of them, ORF8, with its molecular function predicted to encode ribonuclease III activity and RNA binding, is considered the most interesting candidate gene.

分类号： Q94

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