Expression of lactate dehydrogenase is induced during hypoxia via HIF-1 in the mud crab Scylla paramamosain

文献类型： 外文期刊

第一作者： Xie, Jiawei

作者： Xie, Jiawei;Cheng, Changhong;Jie, Yukun;Ma, Hongling;Feng, Juan;Su, Youlu;Deng, Yiqin;Xu, Haidong;Guo, Zhixun;Xie, Jiawei;Jie, Yukun;Guo, Zhixun

作者机构：

关键词： Scylla paramamosain; Hypoxia-inducible factor-1 alpha; Lactate dehydrogenase; Hypoxia; Gene silencing

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY （ 影响因子：3.228； 五年影响因子：3.289 ）

ISSN： 1532-0456

年卷期： 2019 年 225 卷

页码：

收录情况： SCI

摘要： Lactate dehydrogenase (LDH) is a key enzyme involved in anaerobic metabolism in most organisms. In the present study, we determined the structure and function of LDH sequence in Scylla paramamosain (SpLDH) by gene cloning, expression and RNA interference techniques in order to explore the genetic characteristics of LDH and its relationship with HIF-1 during hypoxia. The full-length cDNA was 1453 bp with an open reading frame (ORF) of 996 bp, and encoded a polypeptide of 332 amino acids. Homology analysis showed that the SpLDH gene is highly similar to arthropods. The SpLDH transcript increased after hypoxia in all tested tissues. The silencing of HIF-1 blocked the increase in LDH mRNA and activity, which were induced by hypoxia in gill and muscle tissues. Our results indicated that SpLDH expression was regulated transcriptionally by HIF-1.

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