文献类型： 外文期刊
第一作者： Xue, Junxin
作者： Xue, Junxin;Jiang, Wei;Wang, Quan;Xue, Junxin;Huang, Kehe;Xue, Junxin;Li, Jian;Xiong, Wei;Tian, Zhengan;Zhang, Qiang;Li, Shuqing;Liu, Chunfeng
作者机构：
关键词： protein expression; subcellular localisation; protective effect
期刊名称：FOLIA PARASITOLOGICA （ 影响因子：2.122； 五年影响因子：1.863 ）
ISSN： 0015-5683
年卷期： 2019 年 66 卷
页码：
收录情况： SCI
摘要： Screening and identification of protective antigens are essential for the prevention of infections with Toxoplasma gondii (Nicolle et Manceaux, 1908). In our previous study, T. gondii ribosomal-ubiquitin protein L40 (TgRPL40) was identified as a circulating antigen. However, the function and protective value of TgRPL40 was unknown. In the current study, recombinant TgRPL40 was expressed in Escherichia coli BL21 and antibody was prepared. Western blotting analysis indicated that TgRPL40 was present in circulating antigens and excretory/secretary antigens (ESA). Immunofluorescence and immunoelectron microscopy analysis revealed that TgRPL40 protein is widely distributed in the tachyzoites. Immunisation with recombinant TgRPL40 prolonged the survival of mice infected with tachyzoites. Quantitative real-time polymerase chain reaction analysis showed that immunisation with recombinant TgRPL40 reduced the parasite burden in blood, liver, spleen and brain of mice infected with tachyzoites. These observations indicate that TgRPL40 is a circulating antigen and is an effector of immune protection against acute T. gondii infection.
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