GBSS-BINDING PROTEIN, encoding a CBM48 domain-containing protein, affects rice quality and yield

文献类型： 外文期刊

第一作者： Wang, Wei

作者： Wang, Wei;Wang, Jiayu;Hu, Peisong;Wang, Wei;Wei, Xiangjin;Jiao, Guiai;Chen, Wenqiang;Wu, Yawen;Sheng, Zhonghua;Hu, Shikai;Xie, Lihong;Tang, Shaoqing;Hu, Peisong

作者机构：

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：7.061； 五年影响因子：6.002 ）

ISSN： 1672-9072

年卷期：

页码：

收录情况： SCI

摘要： The percentage of amylose in the endosperm of rice (Oryza sativa) largely determines grain cooking and eating qualities. Granule-bound starch synthase I (GBSSI) and GBSSII are responsible for amylose biosynthesis in the endosperm and leaf, respectively. Here, we identified OsGBP, a rice GBSS-binding protein that interacted with GBSSI and GBSSII in vitro and in vivo. The total starch and amylose contents in osgbp mutants were significantly lower than those of wild type in leaves and grains, resulting in reduced grain weight and quality. The carbohydrate-binding module 48 (CBM48) domain present in the C-terminus of OsGBP is crucial for OsGBP binding to starch. In the osgbp mutant, the extent of GBSSI and GBSSII binding to starch in the leaf and endosperm was significantly lower than wild type. Our data suggest that OsGBP plays an important role in leaf and endosperm starch biosynthesis by mediating the binding of GBSS proteins to developing starch granules. This elucidation of the function of OsGBP enhances our understanding of the molecular basis of starch biosynthesis in rice and contributes information that can be potentially used for the genetic improvement of yield and grain quality.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序