Cryobacterium tepidiphilum sp. nov., isolated from rhizosphere soil of lettuce (var. ramosa Hort.)

文献类型： 外文期刊

第一作者： Wang, Ying

作者： Wang, Ying;Cao, Peng;Sun, Peng;Zhao, Junwei;Sun, Xiujun;Zhang, Ji;Li, Chenxu;Xiang, Wensheng;Wang, Xiangjing;Xiang, Wensheng

作者机构：

关键词： Cryobacterium tepidiphilum sp; nov; Polyphasic analysis; 16S rRNA gene; Rhizosphere soil of lettuce

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.271； 五年影响因子：2.707 ）

ISSN： 0003-6072

年卷期： 2019 年 112 卷 11 期

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收录情况： SCI

摘要： A Gram-stain positive, aerobic, rod-shaped bacterium, designated strain NEAU-85(T), was isolated from rhizosphere soil of lettuce and characterised using a polyphasic approach. Strain NEAU-85(T) was found to be catalase positive, motile and able to grow at between 10 and 30 degrees C. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain NEAU-85(T) belongs to the genus Cryobacterium and is closely related to the type strain Cryobacterium psychrotolerans JCM 13925(T) (98.5%) and also to Leifsonia kafniensis NCCB 100216(T) (97.6%). Multilocus sequence analysis using the concatenated gene sequences of the atpD, dnaK, recA, rpoB, sevY and ychF genes showed that strain NEAU-85(T) belongs to the genus Cryobacterium. The digital DNA-DNA hybridization values revealed that strain NEAU-85(T) is distinct from its close phylogenetic neighbour C. psychrotolerans. The menaquinones were identified as MK-10 and MK-11. The phospholipid profile was found to consist of diphosphatidylglycerol, phosphatidylglycerol, phospholipid, an unidentified glycolipid and an unidentified lipid. The major fatty acids were identified as anteiso-C-15:0, anteiso-C-15:1 and iso-C-16:0. The genomic DNA G + C content of strain NEAU-85(T) was determined to be 68.9 mol%. The DNA-DNA hybridization value between them was less than 70%. On the basis of phenotypic, genotypic and phylogenetic data, strain NEAU-85(T) can be concluded to represent a novel species of the genus Cryobacterium, for which the name Cryobacterium tepidiphilum sp. nov. is proposed. The type strain is NEAU-85(T) (= CCTCC AA 2018035(T) = JCM 32545(T)).

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