Integrin alpha v beta 3 enhances replication of porcine epidemic diarrhea virus on Vero E6 and porcine intestinal epithelial cells

文献类型： 外文期刊

第一作者： Li, Chunqiu

作者： Li, Chunqiu;Su, Mingjun;Guo, Donghua;Wei, Shan;Kong, Fanzhi;Wu, Rui;Sun, Dongbo;Feng, Li;Yin, Baishuang

作者机构：

关键词： Porcine epidemic diarrhea virus; Integrin alpha v beta 3; Virus entry

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2019 年 237 卷

页码：

收录情况： SCI

摘要： The entry mechanism of porcine epidemic diarrhea virus (PEDV) remains unclear, especially the virus receptor. Our previous study revealed a potential correlation between integrin alpha v beta 3 and PEDV infection. In the current study, the effect of overexpression, silencing, antibody inhibition, and co-expression with porcine aminopeptidase N (pAPN) of integrin alpha v beta 3 on PEDV infection was investigated and analyzed in African green monkey Vero E6 cells and porcine intestinal epithelial cells (IECs) using the classical strain CV777 and variant strain HM2017 of PEDV. Integrin alpha v beta 3 significantly enhanced the replication of the classical and variant strains of PEDV in Vero E6 cells and IECs. The integrin av and 133 subunits were both involved in the enhancement of PEDV infection, the Arg Gly Asp peptides targeting integrin alpha v beta 3 significantly inhibited replication of PEDV in Vero E6 cells, and co-expression of integrin alpha v beta 3 with pAPN significantly enhanced replication of PEDV in Vero E6 and BHK-21 cells. These results demonstrate that integrin alpha v beta 3 enhances PEDV replication in Vero E6 cells and IECs. These data provide novel insights into the entry mechanism of PEDV.

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