Identification of mechanisms conferring an enhanced immune response in mice induced by CVC1302-adjuvanted killed serotype O foot-and-mouth virus vaccine

文献类型： 外文期刊

第一作者： Du, Luping

作者： Du, Luping;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Qiao, Xuwen;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Du, Luping;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Qiao, Xuwen;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Du, Luping;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Qiao, Xuwen;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Du, Luping;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Qiao, Xuwen;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Du, Luping;Yu, Xiaoming;Hou, Liting;Zhang, Yuanpeng;Qiao, Xuwen;Hou, Jibo;Chen, Jin;Zheng, Qisheng;Zhang, Dong

作者机构：

关键词： FMDV; CVC1302; Local mechanism; Injection sites; Draining lymph nodes; Mice

期刊名称：VACCINE （ 影响因子：3.641； 五年影响因子：3.816 ）

ISSN： 0264-410X

年卷期： 2019 年 37 卷 43 期

页码：

收录情况： SCI

摘要： The adjuvant CVC1302 was previously shown to efficiently enhance the immunogenicity of killed footand-mouth disease virus (FMDV) in mice and piglets. However, the underlining mechanism of action of CVC1302 remains unclear, especially at local injection sites and draining lymph nodes. Since the FMDV vaccine is administrated intramuscularly in field settings, we studied local immune responses to FMDV following intramuscular injection in mice, and found that CVC1302-adjuvanted killed FMDV (KV-CVC1302) induced secretion of several chemokines in murine muscle tissues, including MCP-1, MIP-1 alpha, and mIP-1 beta. The number of monocytes recruited to the site of injection was significantly higher in mice immunized with KV-CVC1302 compared with mice immunized with killed FMDV alone (MV). iTAQ-based quantitative proteomic assays were additionally employed to explore the molecular mechanisms of CVC1302 action in the draining lymph nodes. A total of 35 proteins were identified as being differentially expressed among the control group, KV-immunized group and KV-CVC1302-immunized group at 10 days post immunization (dpi). Proteins exhibiting differential expression were mainly involved in signal transduction, apoptosis, endocytosis and innate immune responses. Pathway analysis demonstrated that AMPK, phospholipase D, cAMP, Rapt, and MAPK signaling pathways were potentially induced by the immunopotentiator CVC1302. Understanding the local mechanism of CVC1302 action at injection sites and draining lymph nodes will provide new insights into the development of FMDV vaccines. (C) 2019 Elsevier Ltd. All rights reserved.

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