Preparative isolation and purification of B-type fumonisins by using macroporous resin column and high-speed countercurrent chromatography

文献类型： 外文期刊

第一作者： Hu, Junqiang

作者： Hu, Junqiang;Lv, Hui;Hou, Mingxuan;Shi, Jianrong;Gu, Zhenxin;Xu, Jianhong;Hu, Junqiang;Lv, Hui;Hou, Mingxuan;Shi, Jianrong;Gu, Zhenxin;Xu, Jianhong;Hu, Junqiang;Lv, Hui;Hou, Mingxuan;Wang, Gang;Lee, Yin-Won;Hu, Junqiang;Lv, Hui;Hou, Mingxuan;Wang, Gang;Lee, Yin-Won;Hu, Junqiang;Lv, Hui;Hou, Mingxuan;Wang, Gang;Lee, Yin-Won;Hu, Junqiang;Lv, Hui;Hou, Mingxuan;Wang, Gang;Lee, Yin-Won;Hu, Junqiang;Lv, Hui;Hou, Mingxuan;Wang, Gang;Lee, Yin-Won;Shi, Jianrong;Xu, Jianhong

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关键词： Fumonisin; macroporous resin; high-speed countercurrent chromatography

期刊名称：FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT （ 影响因子：3.057； 五年影响因子：2.96 ）

ISSN： 1944-0049

年卷期： 2020 年 37 卷 1 期

页码：

收录情况： SCI

摘要： B-type fumonisins (FBs) are water-soluble mycotoxins produced by Fusarium species, which are mainly found in maize products and threaten food safety. Toxicological studies and quantitative determinations of fumonisins require large amounts of pure toxins, and their high prices limit progress in FBs research. In this study, we used a macroporous resin column combined with high-speed countercurrent chromatography to separate large quantities of FBs. A fermented rice culture was extracted with 75% methanol. The dynamic adsorption capacity of FBs on XAD-2 resin was 27.5 mg/g resin at 25 degrees C, pH 4.0, and then the FBs were desorbed with 60% methanol. The crude FBs were further purified using a biphasic system consisting of n-heptane/n-butanol/methanol/water (2:4:1:4, v/v/v/v). The method yielded 1.55 g of FB1 and 0.55 g of FB3 with purities of 96.8% and 95.6%, respectively, from 1 kg of rice culture, and the final overall yield of FBs was 74.8%.

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