文献类型： 外文期刊
第一作者： Sun, Lili
作者： Sun, Lili;Zhang, Liguo;Zhang, Xiaofei;Chen, Lingling;Bu, Zhigang;Chang, Ying;Wang, Fude;Mu, Yunhui;Chen, Lixin;Guo, Li;Zhang, Xiaofei;Chen, Lingling;Chang, Ying;Wang, Fude;Bu, Zhigang
作者机构：
关键词： Gene clone; Dryopteris fragrans; Transformation; Cinnamate 4-hydroxylase; Overexpression
期刊名称：BANGLADESH JOURNAL OF BOTANY （ 影响因子：0.308； 五年影响因子：0.348 ）
ISSN： 0253-5416
年卷期： 2019 年 48 卷 3 期
页码：
收录情况： SCI
摘要： The enzyme cinnamate 4-hydroxylase (C4H, EC 1.14.13.11), which belongs to the cytochrome P450 family, is involved in the phenylpropanoid pathway. Using RACE, full-length cDNA consisting of 2063 nucleotides encoding C4H (designated DfC4H) was isolated from Dryopteris fragrans (L.) Schott. A number of 1527 of nucleotides composed the complete open reading frame (ORF) of the cDNA (GenBank Accession Number KF830705.2). A protein that consisted of 508 amino acids with a calculated molecular mass of 58.1908 kDa was predicted to be encoded by this ORF. The overexpression of DfC4H was observed to increase significantly p-coumaric acid and anthocyanin levels in transgenic Arabidopsis thaliana.
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