Deep genotyping of the gene GmSNAP facilitates pyramiding resistance to cyst nematode in soybean

文献类型： 外文期刊

第一作者： Tian, Yu

作者： Tian, Yu;Liu, Bo;Shi, Xuehui;Guan, Rongxia;Li, Ying-hui;Qiu, Li-juan;Reif, Jochen C.

作者机构：

关键词： SCN; alpha-SNAP; Allelic combination; Marker-assisted selection (MAS); Haplotype analysis

期刊名称：CROP JOURNAL （ 影响因子：4.407； 五年影响因子：5.687 ）

ISSN： 2095-5421

年卷期： 2019 年 7 卷 5 期

页码：

收录情况： SCI

摘要： Soybean cyst nematode (SCN) is a highly destructive pathogen. The soybean host genome harbors at least two major genes for resistance (rhg1 and Rhg4), as well as a minor locus (SCN3-11). In the present study, a splicing site in GmSNAP11, the potential causal gene of SCN3-11, was identified by comparison of the GmSNAP11 cDNA sequences generated from resistant and susceptible soybean accessions. The sequence information was used to design a codominant CAPS marker, GmSNAP11-2565, which was used to genotype a panel of 209 soybean accessions varying with respect to SCN resistance. Analyses of the effect of the haplotypes formed by GmSNAP11-2565 and another large-effect (nonsynonymous) locus, GmSNAP11-2307, previously identified in GmSNAP11, revealed linkage disequilibrium (P < 0.0001) between the two loci, suggesting that CmSNAP11-2565 could be used as a marker for GmSNAP11. GmSNAP11-2565 was accordingly used, along with established markers for GmSNAP18 (rhg1) and GmSHMT (Rhg4), to characterize the panel accessions. The mean SCN female index of accessions carrying only the GmSNAP11 allele associated with resistance (20.3%) was higher than that associated with accessions carrying alleles for resistance at both GmSNAP11 and GmSNAP18 (12.4%), while the index for accessions carrying alleles for resistance at all of GmSNAP11, GmSNAP18, and GmSHMT was very low (1.9%). Selection on all three markers was effective for maintaining a high level of resistance to SCN race 3. (C) 2019 Crop Science Society of China and Institute of Crop Science, CARS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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