Molecular identification and detection of Wohlfahrtia magnifica in ovine vulvar myiasis in Gansu, China

文献类型： 外文期刊

第一作者： Li, Youquan

作者： Li, Youquan;Li, Xuan;Liu, Junlong;Liu, Aihong;Guo, Pengfei;Han, Yuan;Shang, Youjun;Guan, Guiquan;Liu, Zhijie;Liu, Guangyuan;Luo, Jianxun;Yin, Hong;Liu, Guangyuan;Luo, Jianxun;Yin, Hong

作者机构：

关键词： Vulvar myiasis; Wohlfahrtia magnifica; Identification; Sheep; PCR; Gansu

期刊名称：TROPICAL ANIMAL HEALTH AND PRODUCTION （ 影响因子：1.559； 五年影响因子：1.681 ）

ISSN： 0049-4747

年卷期： 2019 年 51 卷 8 期

页码：

收录情况： SCI

摘要： Traumatic myiasis causes substantial economic losses to farmers worldwide. In the present study, six flocks of sheep (2261 sheep) were investigated in Gansu, China, and 207 of 552 larvae were genetically characterized based on three genes, including cyt b, EF-1 alpha, and white gene, by polymerase chain reaction and sequence analysis. A survey of sheep in China revealed that the prevalence of vulvar myiasis of six sheep flocks was 5.00% (11/220, Flock1), 4.85% (10/206, Flock2), 4.50% (9/200, Flock3), 5.00% (15/300, Flock4), 4.68% (15/320, Flock5), 0% (0/1015, Flock6), respectively. The sequence and phylogenetic analysis showed that only Wohlfahrtia magnifica was detected in the field samples. This is the first report of ovine vulvar myiasis caused by W. magnifica in Gansu, China. Some prophylactic measures are strongly recommended to reduce the risk of sheep acquiring traumatic myiasis in Gansu, China.

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