No imprinted XIST expression in pigs: biallelic XIST expression in early embryos and random X inactivation in placentas

文献类型： 外文期刊

第一作者： Zou, Huiying

作者： Zou, Huiying;Wang, Yangyang;Xu, Huitao;Zhao, Shanjiang;Pang, Yunwei;Liu, Yan;Hao, Haisheng;Zhao, Xueming;Du, Weihua;Zhu, Huabin;Zou, Huiying;Du, Xuguang;Wang, Jing;Dai, Yunping;Li, Ning;Wu, Sen;Yu, Dawei;Zhao, Yunxuan;Chen, Lei;Wu, Sen

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关键词： Pig; Duroc and Rongchang hybrids; SNPs; No imprinted XIST expression; Biallelic XIST expression; Random XCI

期刊名称：CELLULAR AND MOLECULAR LIFE SCIENCES （ 影响因子：9.261； 五年影响因子：9.073 ）

ISSN： 1420-682X

年卷期： 2019 年 76 卷 22 期

页码：

收录情况： SCI

摘要： Dosage compensation, which is achieved by X-chromosome inactivation (XCI) in female mammals, ensures balanced X-linked gene expression levels between the sexes. Although eutherian mammals commonly display random XCI in embryonic and adult tissues, imprinted XCI has also been identified in extraembryonic tissues of mouse, rat, and cow. Little is known about XCI in pigs. Here, we sequenced the porcine XIST gene and identified an insertion/deletion mutation between Asian- and Western-origin pig breeds. Allele-specific analysis revealed biallelic XIST expression in porcine ICSI blastocysts. To investigate the XCI pattern in porcine placentas, we performed allele-specific RNA sequencing analysis on individuals from reciprocal crosses between Duroc and Rongchang pigs. Our results were the first to reveal that random XCI occurs in the placentas of pigs. Next, we investigated the H3K27me3 histone pattern in porcine blastocysts, showing that only 17-31.8% cells have attained XCI. The hypomethylation status of an important XIST DMR (differentially methylated region) in gametes and early embryos demonstrated that no methylation is pre-deposited on XIST in pigs. Our findings reveal that the XCI regulation mechanism in pigs is different from that in mice and highlight the importance of further study of the mechanisms regulating XCI during early porcine embryo development.

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