Molecular identification and diapause-related functional characterization of a novel dual-specificity kinase gene, MPKL, in Locusta migratoria

文献类型： 外文期刊

第一作者： Hao, Kun

作者： Hao, Kun;Ullah, Hidayat;Jarwar, Aftab Raza;Nong, Xiangqun;Tu, Xiongbing;Zhang, Zehua;Ullah, Hidayat

作者机构：

关键词： diapause induction; dual-specificity kinase; FOXO; horizontal gene transfer; RNAi

期刊名称：FEBS LETTERS （ 影响因子：4.124； 五年影响因子：3.814 ）

ISSN： 0014-5793

年卷期： 2019 年 593 卷 21 期

页码：

收录情况： SCI

摘要： Diapause is an important overwintering strategy enabling Locusta migratoria to survive under stressed conditions. We identified a novel dual-specificity kinase gene that is differentially expressed between long and short day-treated L. migratoria. To determine its function on photoperiodic diapause induction, we cloned the specific gene. Interestingly, phylogenetic analysis shows that this dual-specificity kinase is of the mycetozoa protein kinase-like (MPKL) type and may have been transferred horizontally from Mycetozoa to L. migratoria. RNA interference results confirm that MPKL promotes photoperiodic diapause induction of L. migratoria. Furthermore, MPKL significantly inhibits Akt and FOXO (i.e. forkhead box protein O) phosphorylation levels in ovaries, and also enhances reactive oxygen species, superoxide dismutase and catalase activities, whereas peroxidase activity is decreased under both photoperiodic regimes. The findings of the present study offer insight into the molecular mechanism responsible for dual-specificity kinase-induced diapause in insects.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序