Discrimination of Oviposition Deterrent Volatile beta-Ionone by Odorant-Binding Proteins 1 and 4 in the Whitefly Bemisia tabaci

文献类型： 外文期刊

第一作者： Li, Fengqi

作者： Li, Fengqi;Li, Du;Qu, Cheng;Tian, Jiahui;Luo, Chen;Li, Du;Dewer, Youssef;Yang, Zhen

作者机构：

关键词： Bemisia tabaci; odorant-binding protein; competitive binding; beta-ionone

期刊名称：BIOMOLECULES （ 影响因子：4.879； 五年影响因子：5.362 ）

ISSN：

年卷期： 2019 年 9 卷 10 期

页码：

收录情况： SCI

摘要： The whitefly, Bemisia tabaci, is an important invasive economic pest of agricultural crops worldwide. beta-ionone has a significant oviposition repellent effect against B. tabaci, but the olfactory molecular mechanism of this insect for recognizing beta-ionone is unclear. To clarify the binding properties of odorant-binding proteins (OBPs) with beta-ionone, we performed gene cloning, evolution analysis, bacterial expression, fluorescence competitive binding assay, and molecular docking to study the binding function of OBP1 and OBP4 on beta-ionone. The results showed that after the OBP1 and OBP4 proteins were recombined, the compound beta-ionone exhibited a reduction in the fluorescence binding affinity to <50%, with a dissociation constant of 5.15 and 3.62 mu M for OBP1 and OBP4, respectively. Our data indicate that beta-ionone has high affinity for OBP1 and OBP4, which play a crucial role in the identification of oviposition sites in B. tabaci. The findings of this study suggest that whiteflies employ beta-ionone compound in the selection of the suitable egg-laying sites on host plants during the oviposition behavior.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序