Molecular characterization and functional analysis of two new lysozyme genes from soybean cyst nematode (Heterodera glycines)

文献类型： 外文期刊

第一作者： Wang Ning

作者： Wang Ning;Peng Huan;Liu Shi-ming;Huang Wen-kun;Peng De-liang;Holgado, Ricardo;Liu-Clarke, Jihong

作者机构：

关键词： soybean cyst nematode; lysozyme; Hg-lys1; Hg-lys2; innate defense against bacteria

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2019 年 18 卷 12 期

页码：

收录情况： SCI

摘要： Soybean cyst nematode (SCN, Heterodera glycines (I.)) is one of the most important soil-borne pathogens for soybeans. In plant parasitic nematodes, including SCN, lysozyme plays important roles in the innate defense system. In this study, two new lysozyme genes (Hg-lys1 and Hg-lys2) from SCN were cloned and characterized. The in situ hybridization analyses indicated that the transcripts of both Hg-lys1 and Hg-lys2 accumulated in the intestine of SCN. The qRT-PCR analyses showed that both Hg-lys1 and Hg-lys2 were upregulated after SCN second stage juveniles (J2s) were exposed to the Gram-positive bacteria Bacillus thuringiensis, Bacillus subtilis or Staphylococcus aureus. Knockdown of the identified lysozyme genes by in vitro RNA interference caused a significant decrease in the survival rate of SCN. All of the obtained results indicate that lysozyme is very important in the defense system and survival of SCN.

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