The Verticillium dahliae Sho1-MAPK pathway regulates melanin biosynthesis and is required for cotton infection

文献类型： 外文期刊

第一作者： Li, Jun-Jiao

作者： Li, Jun-Jiao;Zhou, Lei;Yin, Chun-Mei;Zhang, Dan-Dan;Wang, Bao-Li;Song, Jian;Wang, Dan;Chen, Jie-Yin;Dai, Xiao-Feng;Zhou, Lei;Chen, Jie-Yin;Dai, Xiao-Feng;Klosterman, Steven J.;Hu, Xiao-Ping;Subbarao, Krishna, V

作者机构：

期刊名称：ENVIRONMENTAL MICROBIOLOGY （ 影响因子：5.491； 五年影响因子：6.438 ）

ISSN： 1462-2912

年卷期： 2019 年 21 卷 12 期

页码：

收录情况： SCI

摘要： Verticillium dahliae is a soil-borne fungus that causes vascular wilt on numerous plants worldwide. The fungus survives in the soil for up to 14 years by producing melanized microsclerotia. The protective function of melanin in abiotic stresses is well documented. Here, we found that the V. dahliae tetraspan transmembrane protein VdSho1, a homolog of the Saccharomyces cerevisiae Sho1, acts as an osmosensor, and is required for plant penetration and melanin biosynthesis. The deletion mutant Delta Sho1 was incubated on a cellophane membrane substrate that mimics the plant epidermis, revealing that the penetration of Delta Sho1 strain was reduced compared to the wild-type strain. Furthermore, VdSho1 regulates melanin biosynthesis by a signalling mechanism requiring a kinase-kinase signalling module of Vst50-Vst11-Vst7. Strains, Delta Vst50, Delta Vst7 and Delta Vst11 also displayed defective penetration and melanin production like the Delta Sho1 strain. Defects in penetration and melanin production in Delta Sho1 were restored by overexpression of Vst50, suggesting that Vst50 lies downstream of VdSho1 in the regulatory pathway governing penetration and melanin biosynthesis. Data analyses revealed that the transmembrane portion of VdSho1 was essential for both membrane penetration and melanin production. This study demonstrates that Vst50-Vst11-Vst7 module regulates VdSho1-mediated plant penetration and melanin production in V. dahliae, contributing to virulence.

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