Use of TRV-mediated VIGS for functional genomics research in citrus

文献类型： 外文期刊

第一作者： Wang, Fusheng

作者： Wang, Fusheng;Xu, Yuanyuan;Liu, Xiaona;Shen, Wanxia;Zhu, Shiping;Zhao, Xiaochun;Wang, Fusheng;Xu, Yuanyuan;Liu, Xiaona;Shen, Wanxia;Zhu, Shiping;Zhao, Xiaochun;Wang, Fusheng

作者机构：

关键词： TRV-mediated VIGS; Vacuum infiltration; Gene silencing; Citrus

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 2019 年 139 卷 3 期

页码：

收录情况： SCI

摘要： Virus induced gene silencing (VIGS) is a powerful tool for identifying gene functions in plants. Tobacco rattle virus (TRV)mediated VIGS has been reported for silencing of endogenous genes in many plants. In citrus, analysis of gene function is mainly relied on the table genetic transformation, even though this technique is low-efficiency and time-consuming. In this study, a TRV-mediated VIGS was successfully applied for verification of gene function in citrus. A reporter gene, Magnesium chelatase subunit I (ChlI) from C. grandis was constructed into TRV2 vector to induce gene silencing with visible photobleaching symptom. TRV VIGS induced photobleaching symptom was observed on the leaves of Nicotiana benthamiana and Citrus after infiltration. The susceptibility to VIGS vector was found to be genotype dependent. Mexican lemon is the most susceptible one. The chlorosis area per leaf was 65.5% of the whole leaf area. GXSTY pummelo exhibited less susceptible to VIGS with only tiny patches of chlorosis appeared on the leaves of treated plants. The parameters were optimized to improve the efficiency. The tiny young leaves and half expanded leaves were more susceptible to VIGS infection. Young seedlings were highly susceptible to vacuum infiltration. The expression of fluorescence from GFP reporter gene was observed in the young seedlings of GXSTY after infiltration with TRV. Close to 100% of infiltration efficiency was achieved by vacuum infiltration. The results indicated that TRV mediated VIGS with vacuum infiltration could be an ideal tool for gene function studies in citrus.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序