The bZIP transcription factor Afap1 mediates the oxidative stress response and aflatoxin biosynthesis in Aspergillus flavus

文献类型： 外文期刊

第一作者： Guan, Xuanli

作者： Guan, Xuanli;Zhao, Yueju;Liu, Xiao;Shang, Bo;Xing, Fuguo;Zhou, Lu;Wang, Yan;Zhang, Chushu;Liu, Yang;Zhang, Chushu;Bhatnagar, Deepak

作者机构：

关键词： Aflatoxin; Oxidative stress; Afap1; Aspergillus flavus

期刊名称：REVISTA ARGENTINA DE MICROBIOLOGIA （ 影响因子：1.852； 五年影响因子：1.667 ）

ISSN： 0325-7541

年卷期： 2019 年 51 卷 4 期

页码：

收录情况： SCI

摘要： Aflatoxin is a carcinogenic secondary metabolite produced mainly by Aspergillus flavus and Aspergillus parasiticus, which can seriously endanger the health of humans and animals. Oxidative stress is a common defense response, and it is known that reactive oxygen species (ROS) can induce the synthesis of a series of secondary metabolites, including aflatoxin. By using mutants lacking the afap 1 gene, the role of afap 1 gene in oxidative stress and aflatoxin synthesis was assessed. The growth of the mutant strains was significantly inhibited by the increase in the concentration of H2O2, inhibition was complete at 40 mmol/l. However, in the quantitative analysis by HPLC, the concentration of AFB(1) increased with the increased H2O2 until 10 mmol/l. Following an analysis based on the information provided by the NCBI BLAST analysis, it was assumed that Afap1, a basic leucine zipper (bZIP) transcription factor, was associated with the oxidative stress in this fungus. Treatment with 5 mmol/l H2O2 completely inhibited the growth of the mutant strains in afap 1 but did not affect the growth of the CA14PTs strain (non-mutant strain). In addition, the concentration of AFB 1 in the mutant strains was approximately 1/4 of that observed in the CA14PTs strain. These results suggested that Afap1 plays a key role in the regulation of oxidative stress and aflatoxin production in A. flavus. (C) 2018 Published by Elsevier Espana, S.L.U. on behalf of Asociacion Argentina de Microbiologia.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序