Antibacterial mechanism of Tetrastigma hemsleyanum Diels et Gilg's polysaccharides by metabolomics based on HPLC/MS

文献类型： 外文期刊

第一作者： Chen, Xiao

作者： Chen, Xiao;Tao, Lan;Ru, Yi;Wang, Jian;Guo, Longhua;Lin, Zhenyu;Qiu, Bin;Weng, Shaohuang;Chen, Zhou;Pan, Wei

作者机构：

关键词： Anti-bacterial activity; Metabonomics; Polysaccharides; Tetrastigma hemsleyanum Diels et Gilg

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2019 年 140 卷

页码：

收录情况： SCI

摘要： Tetrastigma hemsleyanum Diels et Gilg (THDG) is used as a Chinese traditional anti-inflammatory medicine for about thousands of years. In this work, Tetrastigma hemsleyanum Diels et Gilg's polysaccharide (TP) can inhibit E. coli's growth in initial dosing period. Compared with the antibacterial effect of Achyranthe's polysaccharide (AP) from their metabolic profile, it's obviously that their metabolic sites for E. coli were inconsistent. Moreover, TP could not only increase the level of fructose-6-phosphate (F6P), decrease the level of fructose-1,6-diphosphate (FBP), but also charge the amount of the two differential metabolic with the change of the concentration and the dosing time. Actually, F6P could transform into FBP by catalyze of 6-phosphofructokinase-1(6-PFK-1), which is an important process in glycolysis. Furthermore, FBP was considered have positively correlated with E. coifs growth rate. Therefore, TP can inhibit the E. coli's proliferation by interfering with the process for glycolysis and gluconeogenesis. Based on the experimental result, we proposed a new mouthwash method to evaluate the anti-bacterial activity. Compared with AP, TP can inhibit the E. coli's growth within 2 h with a low concentration (0.5%) and a short dosing time (5 min). This study extends the applications of THDG and establishes a new assessment method for the pharmacology activity of Chinese herbal medicine. (C) 2019 Elsevier B.V. All rights reserved.

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