Odorant-Binding Protein HvarOBP5 in Ladybird Hippodamia variegata Regulates the Perception of Semiochemicals from Preys and Habitat Plants

文献类型： 外文期刊

第一作者： Tang, Haoyu

作者： Tang, Haoyu;Xie, Jiaoxin;Liu, Jingtao;Khashaveh, Adel;Liu, Xiaoxu;Yi, Chaoqun;Zhao, Danyang;Zhang, Yongjun;Tang, Haoyu;Liu, Xiaoxu;He, Lin;Tang, Haoyu;Xie, Jiaoxin;Sun, Yang;Xie, Jiaoxin;Yi, Chaoqun

作者机构：

关键词： Hippodamia variegata; odorant-binding protein; binding profiles; electrophysiological response; tendency behavior; RNA interference

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 2 期

页码：

收录情况： SCI

摘要： A total of 12 OBPs were identified in the antennae of ladybird Hippodamia variegata. HvarOBP1/2/5/6/10/11 were highly expressed in adults, whereas HvarOBP3/4/7/8/9/12 had higher expression levels in larvae. In adults, HvarOBP1/2/4/5/6/7/ 8/11/12 were highly expressed in antennae. Among these, recombinant HvarOBP5 strong bound with (E)-beta-farnesene (E beta F), (R)-(+)-limonene, (E,E)-4,8,12-trimethyltrideca-l,3,7,11-tetraene (TMTT), (E)-4,8-dimethyl-1,3,7-nonatriene (DMNT), hexyl hexanoate, and geranyl acetate. Molecular docking indicated that Leu42, Lys43, and His64 were the key binding sites of HvarOBP5. All six ligands evoked electroantennography (EAG) responses in ladybirds. Moreover, (R)-(+)-limonene and hexyl hexanoate were attractive to both sexes. After RNA interference for 72 h, the EAGs of dsRNA-injected ladybirds to DMNT and hexyl hexanoate were significantly decreased by 73.8 and 78.6%, respectively. Both dsRNA-injected males and females showed significantly lower behavioral preferences for DMNT and hexyl hexanoate. These findings suggest that HvarOBP5 in H. variegata plays an important role in the perception of semiochemical cues from preys and habitat plants.

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