Translatome and Transcriptome Analyses Reveal the Mechanism that Underlies the Enhancement of Salt Stress by the Small Peptide Ospep5 in Plants

文献类型： 外文期刊

第一作者： Wang, Jinyan

作者： Wang, Jinyan;Li, Yang;Li, Mingyue;Zhang, Wenting;Ling, Xitie;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Wang, Jinyan;Li, Yang;Li, Mingyue;Zhang, Wenting;Ling, Xitie;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Wang, Jinyan;Chen, Tianzi;Guo, Dongshu;Yang, Yuwen;Liu, Qing;Zhang, Baolong;Zheng, Zhongbing;Zhang, Baolong;Liu, Qing;Lu, Yaping;Hua, Kai

作者机构：

关键词： translatome; transcriptome; peptidome; small peptides; rice salt tolerance

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2024 年 72 卷 8 期

页码：

收录情况： SCI

摘要： Salt stress significantly impedes plant growth and the crop yield. This study utilized de novo transcriptome assembly and ribosome profiling to explore mRNA translation's role in rice salt tolerance. We identified unrecognized translated open reading frames (ORFs), including 42 upstream transcripts and 86 unannotated transcripts. A noteworthy discovery was the role of a small ORF, Ospep5, in conferring salt tolerance. Overexpression of Ospep5 in plants increased salt tolerance, while its absence led to heightened sensitivity. This hypothesis was corroborated by the findings that exogenous application of the synthetic small peptide Ospep5 bolstered salt tolerance in both rice and Arabidopsis. We found that the mechanism underpinning the Ospep5-mediated salt tolerance involves the maintenance of intracellular Na+/K+ homeostasis, facilitated by upregulation of high-affinity potassium transporters (HKT) and Na+/H+ exchangers (SOS1). Furthermore, a comprehensive multiomics approach, particularly ribosome profiling, is instrumental in uncovering unannotated ORFs and elucidating their functions in plant stress responses.

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