miRNA-seq of Echinococcus multilocularis Extracellular Vesicles and Immunomodulatory Effects of miR-4989

文献类型： 外文期刊

第一作者： Ding, Juntao

作者： Ding, Juntao;He, Guitian;He, Guitian;Wu, Jin'en;Yang, Jing;Guo, Xiaola;Zheng, Yadong;Yang, Xing;Wang, Ying;Kandil, Omnia M.;Kutyrev, Ivan;Ayaz, Mazhar;Zheng, Yadong

作者机构：

关键词： Echinococcus multilocularis; emu-miR-4989-3p; extracellular vesicles; macrophage; miRNA

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：5.64； 五年影响因子：6.32 ）

ISSN： 1664-302X

年卷期： 2019 年 10 卷

页码：

收录情况： SCI

摘要： Alveolar echinococcosis caused by Echinococcus multilocularis is an important zoonotic disease. In the infected mice, emu-miR-4989-3p is present in sera, but its role remains unknown. Using high-throughput sequencing and qPCR, emu-miR-4989-3p was herein confirmed to be encapsulated into E. multilocularis extracellular vesicles. In the transfected macrophages, emu-miR-4989-3p was demonstrated to significantly inhibit NO production compared to the control (p < 0.05). Moreover, transfection of emu-miR-4989-3p also gave rise to the increased expression of TNF-alpha (p < 0.01). Furthermore, emu-miR-4989-3p induced the dysregulation of several key components in the LPS/TLR4 signaling pathway compared with the control, especially TLR4 and NF-kappa B that both were upregulated. Conversely, the NO production and the expression of TNF-alpha, TLR4 and NF-kappa B tended to be increased and decreased in the mimics-transfected cells upon emu-miR-4989-3p low expression, respectively. These results suggest that emu-miR-4989-3p is one of 'virulence' factors encapsulated into the extracellular vesicles, potentially playing a role in the pathogenesis of E. multilocularis.

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