A new method for determination of campesterol, stigmasterol and beta-sitosterol in edible oils by supercritical fluid chromatography

文献类型： 外文期刊

第一作者： Qi, Ningli

作者： Qi, Ningli;Yang, Chunliang;Liu, Yijun;Liao, Liangkun;Gong, Xiao

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关键词： campesterol; stigmasterol; beta-sitosterol; edible oils; supercritical fluid chromatography

期刊名称：JOURNAL OF FOOD AND NUTRITION RESEARCH （ 影响因子：1.333； 五年影响因子：1.189 ）

ISSN： 1336-8672

年卷期： 2019 年 58 卷 4 期

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收录情况： SCI

摘要： This study aimed to develop a rapid method for separating three major phytosterols (stigmasterol, campesterol and beta-sitosterol) in edible oils by supercritical fluid chromatography using the ultra-performance convergence chromatography system. The samples were prepared by alkaline saponification followed by n-hexane extraction and then separated on a high strength silica C-18 column with selectivity for bases using acetonitrile-methanol (50:50) as a co-solvent with a gradient elution (98:2 to 80:20) and UV-spectrophotometric detection at 210 nm. The optimized method yielded a linear calibration curve ranging from 0.07 ng.ml(-1) to 200 ng.ml(-1) for stigmasterol, from 0.12 ng.ml(-1) to 200 ng.ml(-1) for campesterol and from 0.06 ng.ml(-1) to 200 ng.ml(-1) for beta-sitosterol. The limit of detection and limit of quantification of the phytosterols were 20-42 ng.ml(-1) and 75-117 ng.ml(-1), respectively. The recovery rates ranged from 96.4 % to 101.2 % with relative standard deviations of 1.7-3.8 %, depending on the sterol type and the specific sample. The developed method is simple and sensitive, as demonstrated by its successful use for phytosterol characterization in edible oils.

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