A SYBR green I-based quantitative RT-PCR assay for bovine ephemeral fever virus and its utility for evaluating viral kinetics in cattle
文献类型： 外文期刊
第一作者： Gao, Shandian
作者： Gao, Shandian;Du, Junzheng;Tian, Zhancheng;Niu, Qingli;Huang, Dexuan;Wang, Jidong;Luo, Jianxun;Liu, Guangyuan;Yin, Hong;Yin, Hong
作者机构：
关键词： bovine ephemeral fever virus; infection kinetics; quantitative RT-PCR
期刊名称：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION （ 影响因子：1.279； 五年影响因子：1.522 ）
ISSN： 1040-6387
年卷期： 2020 年 32 卷 1 期
页码：
收录情况： SCI
摘要： We developed a SYBR green I-based reverse-transcription quantitative PCR (RT-qPCR) assay for bovine ephemeral fever virus (BEFV). Analytical sensitivity of the assay was similar to 100 times higher than conventional RT-PCR. The precision of the RT-qPCR established for RNA standards was high, with intra-assay and inter-assay coefficients of variation of 0.23-0.89% and 0.23-1.02%, respectively. The test was highly specific for BEFV strains, with no cross-reactivity with other viruses of veterinary significance. The assay detected BEFV RNA as early as 1 d post-infection (dpi) and up to 7-8 dpi in the blood samples of experimentally infected cattle. The most stable reference gene, peptidylprolyl isomerase A (PPIA), was selected for the quantification of BEFV. Viral RNA loads reached peak level at 3-5 dpi and then decreased rapidly through 7-8 dpi. Our assay provides a reliable approach for the detection of BEFV in the early infection stage and for use in the profiling of BEFV kinetics in vivo.
分类号：
- 相关文献
作者其他论文 更多>>
-
A vesicular stomatitis virus-based African swine fever vaccine prototype effectively induced robust immune responses in mice following a single-dose immunization
作者：Ma, Yunyun;Shao, Junjun;Liu, Wei;Gao, Shandian;Peng, Decai;Miao, Chun;Yang, Sicheng;Hou, Zhuo;Zhou, Guangqing;Chang, Huiyun;Qi, Xuefeng
关键词：African swine fever virus; vaccine prototypes; vesicular stomatitis virus; safety; immune potency
-
mmu-miR-374b-5p modulated inflammatory factors via downregulation of C/EBP β/NF-κB signaling in Kupffer cells during Echinococcus multilocularis infection
作者：Pu, Guiting;Li, Yanping;Liu, Tingli;Li, Hong;Wang, Liqun;Chen, Guoliang;Cao, Shanling;Yin, Hong;Amuda, Tharheer Oluwashola;Guo, Xiaola;Luo, Xuenong;Yin, Hong;Luo, Xuenong
关键词：Echinococcus multilocularis; miR-374b-5p; Inflammatory factors; C/EBP beta; NF-kappa B
-
Establishment of an ELISA Based on a Recombinant Antigenic Protein Containing Multiple Prominent Epitopes for Detection of African Swine Fever Virus Antibodies
作者：Afayibo, Dosseh Jean Apotre;Zhang, Zhonghui;Sun, Hualin;Fu, Jingsheng;Zhao, Yaru;Amuda, Tharheer Oluwashola;Wu, Mengli;Du, Junzheng;Guan, Guiquan;Niu, Qingli;Yang, Jifei;Yin, Hong;Yin, Hong
关键词：African swine fever virus; indirect ELISA; B-cell epitopes; pig; endemic
-
Simultaneous detection of seven bacterial pathogens transmitted by flies using the reverse line blot hybridization assay
作者：Ma, Yonghua;Sun, Xiaolin;Li, Yuanyuan;Gou, Huitian;Wang, Zexiang;Song, Beibei;Niu, Qingli
关键词：Reverse line blot; Fly-borne; Hybridization assay; Bacterial pathogens; Probes
-
Discovery and vertical transmission analysis of Dabieshan Tick Virus in Haemaphysalis longicornis ticks from Chengde, China
作者：Xu, Xiaofeng;Gao, Zhihua;Wu, Youhong;Yang, Xiaolong;Chen, Ze;Yin, Hong;Ren, Qiaoyun;Zhang, Jie;Liu, Yongsheng;Yang, Shunli;Bayasgalan, Chimedtseren;Tserendorj, Ariunaa
关键词：Haemaphysalis longicornis; Dabieshan Tick Virus; ticks; transstadial transmission; transovarial transmission; Hebei Province
-
Amendment of straw with decomposing inoculants benefits the ecosystem carbon budget and carbon footprint in a subtropical wheat cropping field
作者：Ji, Cheng;Wang, Jidong;Xu, Cong;Yuan, Jie;Liang, Dong;Wang, Lei;Ning, Yunwang;Zhang, Yongchun;Ji, Cheng;Wang, Jidong;Xu, Cong;Zhang, Yongchun;Gu, Yian;Zhou, Jie
关键词：
-
Wheat straw and microbial inoculants have an additive effect on N2O emissions by changing microbial functional groups
作者：Ji, Cheng;Wang, Jidong;Sun, Yuxiang;Xu, Cong;Zhong, Yuehua;Ning, Yunwang;Zhang, Hui;Zhang, Yongchun;Ji, Cheng;Wang, Jidong;Xu, Cong;Zhang, Hui;Zhang, Yongchun;Wang, Jidong;Zhang, Yongchun;Sun, Yuxiang;Zhou, Jie;Chen, Yinglong;Chen, Yinglong
关键词：microbial gene abundance; microbial inoculant; nitrous oxide; straw
