Rational design of a lysosomal-targeted ratiometric two-photon fluorescent probe for imaging hydrogen polysulfides in live cells

文献类型： 外文期刊

第一作者： Han, Qingxin

作者： Han, Qingxin;Liu, Xuan;Wang, Xuechuan;Yin, Ruojun;Jiang, Huie;Ru, Jiaxi;Han, Qingxin;Liu, Weisheng

作者机构：

关键词： Lysosome-targeting; Ratiometric; Two-photon; Fluorescent probe; Hydrogen polysulfide

期刊名称：DYES AND PIGMENTS （ 影响因子：4.889； 五年影响因子：4.016 ）

ISSN： 0143-7208

年卷期： 2020 年 173 卷

页码：

收录情况： SCI

摘要： Hydrogen polysulfides (H2Sn, n > 1) play important roles in H2S associated physiological functions and pathological processes and H2S2 is regarded to be a newfound potent signal transducer. But fluorescent probes for rapid, sensitive, and selective detecting of H2Sn are still lacking, above all the lysosomal-targeted ones. In this work, by integrating the alkylmorpholine group and 2-fluoro-5-nitrobenzoyl- group into the molecular structure of 1,8-naphthalimide based fluorophore, a new lysosome-targetable ratiometric two-photon fluorescent probe for H2Sn has been designed and synthesized. The probe showed good stability, short response time (5 min), high selectivity and sensitivity (LOD = 10 nM), broad linear range (0-25 mu M), admirable two-photon absorption cross sections (Phi(max) = 324 GM), fine membrane permeability and biocompatibility, and as well as the lysosome targeting ability. Moreover, this work affords a promising approach for detecting and imaging of lysosomal H2Sn, and therefore potentially applied in the study of H2Sn-related biology and pathology.

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