Profile and development of microsatellite primers for Acanthogobius ommaturus based on high-throughput sequencing technology

文献类型： 外文期刊

第一作者： Song Chenyu

作者： Song Chenyu;Feng Ziyi;Sun Zhicheng;Song Na;Liu Lu;Li Chunhou;Gao Tianxiang

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关键词： microsatellite; Acanthogobius ommaturus; high-throughput sequencing; polymorphic sites

期刊名称：JOURNAL OF OCEANOLOGY AND LIMNOLOGY （ 影响因子：1.265； 五年影响因子：1.276 ）

ISSN： 2096-5508

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收录情况： SCI

摘要： Acanthogobius ommaturus, a fish species of the Family Gobiidae, is a marine commercial fish perched on the bottom of seawater. In this study, Illumina high-throughput sequencing technology was applied to obtain the candidate microsatellite markers of A. ommaturus. A total of 4 746 microsatellite-rich fragments were found, of which 4 542 microsatellites are with primer fragments, containing 971 dinucleotide sequences, 2 643 trinucleotide sequences, 569 tetranucleotide sequences, 406 pentanucleotide sequences, and 212 hexanucleotide sequences. Based on the results of high-throughput sequencing, a total of 141 pairs of the microsatellite primers were designed and screened. And then 24 polymorphic primers were finally obtained by polyacrylamide gel electrophoresis. In total, 271 alleles were detected in the 24 pairs of primers. The number of alleles for different primers ranged from 5 to 19. The average number of effective alleles (N-a) was 11.292; the average observed heterozygosity (H-o) of the 24 pairs of primers was 0.665, the average expected heterozygosity (H-e) was 0.880, and the average polymorphic information content was 0.846. All sites were highly polymorphic (PIC>0.50).

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