A new adaptation for in vitro co-culture of single porcine parthenogenetic embryos using feeder cells

文献类型： 外文期刊

第一作者： Zhang, L.

作者： Zhang, L.;Lin, Z.;Hua, Z.;Zheng, X.;Xiao, H.;Hua, W.;Ren, H.;Zhu, Z.;Bi, Y.;Molenaar, A.

作者机构： Hubei Acad Agr Sci, Key Lab Anim Embryo Engn & Mol Breeding Hubei Pro, Inst Anim Sci & Vet Med, Wuhan 430064, Hubei, Peoples R China;AgReserach Ltd, Grasslands Res Ctr, Private Bag 11008, Palmerston North 4442, New Zealand

关键词： feeder cells; porcine; embryos; in vitro

期刊名称：POLISH JOURNAL OF VETERINARY SCIENCES （ 2020影响因子：0.821； 五年影响因子：1.005 ）

ISSN： 1505-1773

年卷期： 2019 年 22 卷 4 期

页码：

收录情况： SCI

摘要： Feeder cells can promote cell proliferation and help overcome the developmental arrest of early embryos by producing growth factors. The objective of this study was to evaluate the effects of feeder cells on the development of all single porcine parthenogenetic embryos in vitro. Firstly, we showed that the cleavage and blastocyst formation rate of all single procine parthenogenetic embryos co-cultured with feeder cells increased in contrast to those cultured without feeder cells (p<0.05). However, no statistically significant differences were observed between the blastocyst formation rate in the embryos co-cultured with 3 different kinds feeder cells namely oviduct epithelial feeder cells, granulose feeder cells and porcine fetal fibroblast feeder cells (p>0.05). Secondly, highly significant differences were observed between the cleavage and blastocyst formation rate (p<0.05) when the embryos were co-cultured with oviduct epithelial feeder cells in different volume drops ranging from 3 to 20 mu L and the cleavage rate were the highest when cultured in 5 mu L drops. Thirdly, the tempospacial pattern of the development of single embryos co-cultured with oviduct epithelial feeder cells was consistent with that of traditional multi-embryo culture, indicating that the co-culturing does not affect the developmental competence of the porcine parthenogenetic embryos. Finally, highly significant diffe-rences were observed between the cleavage and blastocyst formation rate with and without zona pellucida in vitro (p<0.05). In this study, a new adaption of in vitro co-culture of single porcine parthenogenetic embryos using feeder cells has been successfully established and this will facilitate further investigations to discover the mechanistic mode of developmental arrest of porcine embryos.

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