Implication of Broadly Neutralizing Bovine Monoclonal Antibodies in the Development of an Enzyme-Linked Immunosorbent Assay for Detecting Neutralizing Antibodies against Foot-and-Mouth Disease Virus Serotype O

文献类型： 外文期刊

第一作者： Cao, Yimei

作者： Cao, Yimei;Li, Kun;Wang, Sheng;Fu, Yuanfang;Sun, Pu;Li, Pinghua;Bai, Xingwen;Zhang, Jing;Ma, Xueqing;Xing, Xiangchuan;Zhou, Shasha;Bao, Huifang;Li, Dong;Chen, Yingli;Li, Zhiyong;Lu, Zengjun;Liu, Zaixin;Xing, Xiangchuan

作者机构：

关键词： foot-and-mouth disease virus; broadly neutralizing bovine monoclonal antibody; blocking ELISA; neutralizing antibody; serotype O

期刊名称：JOURNAL OF CLINICAL MICROBIOLOGY （ 影响因子：5.948； 五年影响因子：5.345 ）

ISSN： 0095-1137

年卷期： 2019 年 57 卷 12 期

页码：

收录情况： SCI

摘要： Vaccination with inactivated vaccines is still the main measure to control foot-and-mouth disease (FMD) in areas where the disease is endemic, and the level of neutralizing antibody in vaccinated animals is directly related to their protection against virus challenge. Currently, neutralizing antibody is mainly detected using the virus neutralization test (VNT) based on cell culture, which is laborious and time-consuming and requires restrictive biocontainment facilities. In this study, two broadly neutralizing antibodies (bnAbs), E46 and F128, were successfully produced using techniques for the isolation of single B cells from peripheral blood mononuclear cells (PBMCs) from bovines sequentially immunized with three topotypes of foot-and-mouth disease virus (FMDV) serotype O. Based on these bnAbs, a blocking enzyme-linked immunosorbent assay (ELISA) for detecting neutralizing antibodies (NA-ELISA) against FMDV serotype O was developed. The specificity and sensitivity of the test were estimated to be 99.21% and 100%, respectively. A significant correlation (P < 0.01) was observed between the NA-ELISA titers and the VNT titers for all sera from vaccinated animals and for all tested strains, suggesting that the NA-ELISA could detect neutralizing antibodies against FMDV serotype O strains of wide antigenic and molecular diversity and could be used for the evaluation of protective immunity.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序