Constitutive expression of a membrane-bound NAC transcription factor AmNTL1 from a desert shrub Ammopiptanthus mongolicus enhances abiotic stress tolerance of transgenic Arabidopsis

文献类型： 外文期刊

第一作者： Tang, Kuangang

作者： Tang, Kuangang;Zhang, Yanxia;Ren, Meiyan;Xue, Min;Zhang, Min;Pang, Xinyue;Wang, Maoyan;Tang, Kuangang

作者机构：

关键词： Abiotic stress; Ammopiptanthus mongolicus; Gene expression; NTL transcription factor; Transgenic Arabidopsis

期刊名称：SOUTH AFRICAN JOURNAL OF BOTANY （ 影响因子：3.1； 五年影响因子：3.1 ）

ISSN： 0254-6299

年卷期： 2023 年 156 卷

页码：

收录情况： SCI

摘要： The NTM1-like (NTL) proteins constitute a subfamily of the NAM, ATAF and CUC (NAC) transcription factor family that regulates diverse biological processes including stress response in plants. Ammopiptanthus mon-golicus is a desert shrub with very high tolerance to harsh environments. Here, an NTL-like gene, AmNTL1, was cloned from this species and its function in abiotic stress tolerance was analyzed. AmNTL1 encodes a canonical NTL protein. The full-length protein was primarily located at the plasma membrane and had trans-activation activity in yeast. The expression of AmNTL1 was differentially induced by cold, salt, drought, and exogenous abscisic acid (ABA) in laboratory-cultured A. mongolicus seedlings. The expression was also induced by the cold winter and spring weather in leaves of the A. mongolicus shrubs naturally growing in the wild and was at moderate levels in lateral roots, young leaves and flower buds of the shrubs. The trans-genic Arabidopsis lines constitutively expressing AmNTL1 showed enhanced tolerance to freezing, chilling, salinity, and osmotic stress as well as chilling-and salt-induced oxidative stresses. The transgenic lines also displayed ABA-sensitive phenotypes at the seed germination stage. These results suggest that AmNTL1 may play an important role in plants coping with abiotic stresses, especially cold injury, through the ABA-depen-dent signaling pathway.(c) 2023 SAAB. Published by Elsevier B.V. All rights reserved.

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