Identification of fungal populations assimilating rice root residue-derived carbon by DNA stable-isotope probing

文献类型： 外文期刊

第一作者： Zhang, Qian

作者： Zhang, Qian;Li, Hui;Wang, Yilun;Guo, Tengfei;Zhou, Wei

作者机构：

关键词： Stable isotope probing (SIP); Rice root residue; C-13; Fungal community

期刊名称：APPLIED SOIL ECOLOGY （ 影响因子：4.046； 五年影响因子：4.884 ）

ISSN： 0929-1393

年卷期： 2020 年 147 卷

页码：

收录情况： SCI

摘要： Determining the underlying mechanisms of microbes having in biogeochemical processes is a research hotspot, but the specific taxa that undergo exogenous carbon (C) utilization remain ambiguous. Soil microcosm was established to identify fungal populations that can utilize root residue-derived C by using DNA-stable isotope probing coupled with Illumina MiSeq-based sequencing of ribosomal markers. Major fungal populations associated with C assimilation were affiliated with phyla Basidiomycota and Ascomycota. A succession of C consumers was observed over time from genera Talaromyces and Masariosphaeria to the more enriched genera, like Bulleromyces and Arnium. Additionally, LEfSe analysis of the enriched C-13 in fungal DNA further showed that fungi in orders Sordariales and Eurotiomycetes, families Lindgomycetaceae, Montagnulaceae and Onygenaceae, and genera Staphylotrichum and Penicillium were key microbes in fresh C utilization in this paddy soil. The results help improving our understanding about the mechanisms of root residue-derived C cycling and emphasizing the importance of this huge amount C utilization.

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