A Marker-Free Bordetella bronchiseptica aroA/bscN Double Deleted Mutant Confers Protection against Lethal Challenge

文献类型： 外文期刊

第一作者： Ai, Weicheng

作者： Ai, Weicheng;Peng, Zhong;Wang, Fei;Zhang, Yue;Xie, Sisi;Liang, Wan;Hua, Lin;Wang, Xiangru;Chen, Huanchun;Wu, Bin;Ai, Weicheng;Peng, Zhong;Wang, Fei;Zhang, Yue;Xie, Sisi;Liang, Wan;Hua, Lin;Wang, Xiangru;Chen, Huanchun;Wu, Bin;Liang, Wan

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关键词： Bordetella bronchiseptica; aroA/bscN double deleted mutant; safety; immune efficacy; attenuated live vaccine

期刊名称：VACCINES （ 影响因子：4.422； 五年影响因子：5.513 ）

ISSN：

年卷期： 2019 年 7 卷 4 期

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收录情况： SCI

摘要： Bordetella bronchiseptica is a leading cause of swine respiratory disorders which depict a great threat to well-flourished porcine industry. Vaccination remains an effective way for the prevention of B. bronchiseptica infections, as live B. bronchiseptica vaccines possess many advantages compared to inactivated vaccines and/or sub-unit vaccines, however, their safety is not up to the mark. In present study, we constructed marker-free aroA/bscN double deleted B. bronchiseptica QH09 through two-step homologous recombination strategy. Our data showed that QH09 attenuated virulence to mice compared with the parent aroA deleted B. bronchiseptica QH0814. We also found that QH09 meets the vaccine safety standards, upon challenge in piglets, did not cause any visible clinical signs or lesions on organs. Finally, we demonstrated that vaccination of QH09 activated the systemic as well as the mucosal immunity in pigs and provided protection against lethal bacterial challenge. These findings suggest that the aroA/bscN double deleted B. bronchiseptica QH09 may be an effective vaccine candidate, with safety assurance of animals against B. bronchiseptica infections.

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