Prevalence and genetic diversity of grapevine fabavirus isolates from different grapevine cultivars and regions in China

文献类型： 外文期刊

第一作者： Fan Xu-dong

作者： Fan Xu-dong;Zhang Meng-yan;Zhang Zun-ping;Ren Fang;Hu Guo-jun;Dong Ya-feng

作者机构：

关键词： GFabV; RT-PCR; polyprotein gene; phylogenetic analysis

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2020 年 19 卷 3 期

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收录情况： SCI

摘要： A total of 288 grapevine samples of 61 different grapevine cultivars, collected from 22 provinces and regions, were analyzed by reverse transcription-PCR (RT-PCR) for the presence of grapevine fabavirus (GFabV). PCR detection results showed the incidences of GFabV were 12.8% (30/235) and 48.1% (25/52) in the asymptomatic and symptomatic vines, respectively. The genetic diversity of GFabV isolates was analyzed based on partial nucleotide and encoded amino acid sequences of the RNA1 and RNA2 polyprotein genes. Phylogenetic analyses of the RNA1 and RNA2 gene sequences divided the GFabV isolates into five well-defined groups. Groups 1, 2, and 4 comprised only Chinese isolates. This article represents the first report for the prevalence and genetic diversity of GFabV in grapevines grown in China.

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