Preferential assimilation of NH4+ over NO3- in tea plant associated with genes involved in nitrogen transportation, utilization and catechins biosynthesis

文献类型： 外文期刊

第一作者： Tang, Dandan

作者： Tang, Dandan;Liu, Mei-Ya;Zhang, Qunfeng;Ma, Lifeng;Shi, Yuanzhi;Ruan, Jianyun;Tang, Dandan

作者机构：

关键词： (NH4+)-N-15; (NO3-)-N-15; Assimilation; Theanine; Catechins; Tea plant

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2020 年 291 卷

页码：

收录情况： SCI

摘要： Physiological effects of ammonium (NH4+) and nitrate (NO3-) on tea have confirmed that tea plants prefer NH4+ as the dominant nitrogen (N) source. To investigate the possible explanations for this preference, studies of (NH4+)-N-15 and (NO3-)-N-15 assimilation using hydroponically grown tea plants were conducted. During the time course of (NH4+)-N-15 and (NO3-)-N-15 assimilation, the absorption of N-15 from (NH4+)-N-15 was more rapid than that from (NO3-)-N-15, as there was a more efficient expression pattern of NH4+ transporters compared with that of NO3- transporters. (NH4+)-N-15-fed tea plants accumulated more N-15 than (NO3-)-N-15 fed plants, which was demonstrated by that genes related to primary N assimilation, like CsNR, CsNiR, CsGDH and CsGOGAT, were more affected by (NH4+)-N-15 than (NO3)-N-15. Markedly higher NH4+ concentrations were observed in (NH4+)-N-15-fed tea roots in comparison with NO3- treatment, whereas tea plants maintained a balanced concentration of NH4+ in tea leaves under both these two N forms. This maintenance was achieved through the increased expression of genes involved in theanine biosynthesis and the inhibition of genes related to catechins derived from phenylpropanoid pathway. The current results suggest that efficient NH4+ transportation, assimilation, and reutilization enables tea plant as an ammonium preferring plant species.

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