Comparative genomic analysis reveals an 'open' pan-genome of African swine fever virus

文献类型： 外文期刊

第一作者： Wang, Liang

作者： Wang, Liang;Zhao, Yuhui;Gao, George F.;Bi, Yuhai;Luo, Yuzi;Qiu, Hua-Ji;Gao, George F.;Bi, Yuhai;Gao, George F.;Gao, George F.

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关键词： accessory genes; African swine fever virus; core genes; evolution; open; pan-genome; taxis-related genes

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：5.005； 五年影响因子：4.622 ）

ISSN： 1865-1674

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收录情况： SCI

摘要： The worldwide transmission of African swine fever virus (ASFV) drastically affects the pig industry and global trade. Development of vaccines is hindered by the lack of knowledge of the genomic characteristics of ASFV. In this study, we developed a pipeline for the de novo assembly of ASFV genome without virus isolation and purification. We then used a comparative genomics approach to systematically study 46 genomes of ASFVs to reveal the genomic characteristics. The analysis revealed that ASFV has an 'open' pan-genome based on both protein-coding genes and intergenic regions. Of the 151-174 genes found in the ASFV strains, only 86 were identified as core genes; the remainder were flexible accessory genes. Notably, 44 of the 86 core genes and 155 of the 324 accessory genes have been functionally annotated according to the known proteins. Interestingly, a dynamic number of taxis-related genes were identified in the accessory genes, and two potential virulence genes were identified in all ASFV isolates. The 'open' pan-genome of ASFV based on gene and intergenic regions reveals its pronounced natural diversity concerning genomic composition and regulation.

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