Detection and molecular characterization of a phytoplasma in Eclipta prostrata in China

文献类型: 外文期刊

第一作者: Chen, Wang

作者: Chen, Wang;Li, Yan;Fang, Xiaoping

作者机构:

关键词: Phytoplasma; Eclipta prostrata; Candidatus Phytoplasma aurantifolia

期刊名称:JOURNAL OF GENERAL PLANT PATHOLOGY ( 影响因子:1.449; 五年影响因子:1.416 )

ISSN: 1345-2630

年卷期: 2020 年 86 卷 1 期

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收录情况: SCI

摘要: Witches' broom and phyllody, associated with phytoplasma infection, were observed on Eclipta prostrata, a common herb throughout the world, in Haikou, Hainan Province, China. Phytoplasma infection was confirmed using polymerase chain reaction and phytoplasma-specific primer pairs P1/P7, R16F2n/R16R2, rp(II)F/rp(II)R and subsequent amplicon sequencing. Based on the partial sequence of 16S rRNA gene and ribosomal protein gene, phylogenetic tree analysis and RFLP analysis of phytoplasma showed that the phytoplasma was a strain related to 'Candidatus Phytoplasma aurantifolia'. To our knowledge, this is the first report of a phytoplasma in E. prostrata worldwide.

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