Identification of soybean drought-tolerant genotypes and loci correlated with agronomic traits contributes new candidate genes for breeding

文献类型： 外文期刊

第一作者： Chen, Limiao

作者： Chen, Limiao;Fang, Yisheng;Chen, Haifeng;Yang, Hongli;Cao, Dong;Hao, Qingnan;Yuan, Songli;Zhang, Chanjuan;Guo, Wei;Chen, Shuilian;Yang, Zhonglu;Shan, Zhihui;Zhang, Xiaojuan;Qiu, Dezhen;Zhou, Xin-An;Chen, Limiao;Fang, Yisheng;Chen, Haifeng;Yang, Hongli;Cao, Dong;Hao, Qingnan;Yuan, Songli;Zhang, Chanjuan;Guo, Wei;Chen, Shuilian;Yang, Zhonglu;Shan, Zhihui;Zhang, Xiaojuan;Qiu, Dezhen;Zhou, Xin-An;Zeng, Kai;Zhang, Hengbin;Zhan, Yong;Li, Xiangyong

作者机构：

关键词： Physiological and agronomic traits; Drought stress; Drought-tolerant index; Soybean; SNPs; Candidate genes

期刊名称：PLANT MOLECULAR BIOLOGY （ 影响因子：4.076； 五年影响因子：4.89 ）

ISSN： 0167-4412

年卷期： 2020 年 102 卷 1-2 期

页码：

收录情况： SCI

摘要： Key message Drought tolerance level of 136 soybean genotypes, the correlations among traits were evaluated, and several important drought-tolerant genotypes, traits, SNPs and genes were possibly useful for soybean genetic breeding. Drought is an adverse environmental factor affecting crops growth, development, and yield. Promising genotypes and genes with improved tolerance to drought are probably effective ways to alleviate the situation. In this study, our main task was to determine drought tolerance level of 136 soybean genotypes, the correlations among physiological and agronomic traits under drought, and drought-tolerant single nucleotide polymorphism (SNPs) and genes. In this study, twenty-six varieties were identified as excellent tolerant genotypes to stress among which S14, S93 and S135 with high drought-tolerant index (DTI > 1.3) and yield (Y > 300 kg). Fourteen varieties were identified as drought-sensitive genotypes, such as S25, S45 and S58, with low drought-tolerant index (DTI < 0.5). 422 SNPs and 302 genes correlated with seed number per plant (SNPP), maturity (M), number of seeds per pod (NSPP), node number of main stem (NNMS), Stem diameter (SD) and pull stem (PS) were detected under well-watered and drought conditions by genome-wide association study (GWAS). Among them, we found SNPs (Chr 3:1758920-1958934) between drought-tolerant and sensitive genotypes. 13 genes (Glyma.03G017800, Glyma.03G018000, Glyma.03G018200, Glyma.03G018400, Glyma.03G018500, Glyma.03G018600, Glyma.03G018700, Glyma.03G018800, Glyma.03G018900, Glyma.03G019000, Glyma.03G019100, Glyma.03G019200, Glyma.03G019300) correlated with NNMS were detected. By qRT-PCR, the expression level of Glyma.03G018000 and Glyma.03G018900 in drought-tolerant varieties was significantly increased, but low or no expression in sensitive varieties under drought stress. This study provides important drought-tolerant genotypes, traits, SNPs and potential genes, possibly useful for soybean genetic breeding.

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