Effects of alpha-ketoglutarate on growth performance, antioxidant capacity and ammonia metabolization against chronic carbonate alkalinity stress in Songpu mirror carp (Cyprinus carpio Songpu)

文献类型： 外文期刊

第一作者： Fan, Ze

作者： Fan, Ze;Peng, Feng;Li, Jinnan;Wu, Di;Zhang, Yuanyuan;Wang, Chang'an;Wang, Liansheng;Xu, Qiyou;Xu, Qiyou

作者机构：

关键词： -ketoglutarate; ammonia metabolization; antioxidant capacity; carbonate alkalinity; growth performance; Songpu mirror carp

期刊名称：AQUACULTURE RESEARCH （ 影响因子：2.082； 五年影响因子：2.415 ）

ISSN： 1355-557X

年卷期： 2020 年 51 卷 5 期

页码：

收录情况： SCI

摘要： A 3 x 2 factorial experiment was conducted to investigate the interaction between carbonate alkalinity and dietary alpha-ketoglutarate (AKG) levels on the growth performance, antioxidant capacity and ammonia metabolization of Songpu mirror carp (Cyprinus carpio Songpu). Each diet (0%, 1% AKG) was randomly allotted to 0 mmol/L, 15 mmol/L, 30 mmol/L carbonate alkalinity groups with three replicate aquaria. The weight gain rate (WGR) significantly increased in the 1% AKG group and significantly decreased with increasing carbonate alkalinity (p < .05). Crude ash was significantly affected by the interaction between carbonate alkalinity and dietary AKG levels and significantly increased in the 1% AKG group (p < .05). A significant interaction between carbonate alkalinity and dietary AKG levels on superoxide dismutase (SOD) activities in hepatopancreas was observed (p < .05). The increasing carbonate alkalinities significantly decreased SOD activities in the hepatopancreas and foregut, catalase (CAT) activities in the hepatopancreas, foregut, midgut and hindgut and glutathione (GSH) contents in the hepatopancreas and foregut (p < .05). Despite AKG addition, the blood ammonia content still increased with increasing carbonate alkalinities. The gene expressions of AQP3, Rhag, Rhcg2 and Na+/K+-ATPase (NKA) in the gills and glutamine synthase (GS) in the brain were significantly upregulated with increasing carbonate alkalinities (p < .05). In the 1% AKG group, GS gene expression significantly upregulated in the brains, whereas AQP3, Rhag, Rhcg2 and NKA gene expressions significantly downregulated in the gills (p < .05). In summary, the 1% AKG addition can enhance the gene expression of ammonia metabolization and improve the antioxidant capacity of Songpu mirror carp with chronic carbonate alkalinity stress.

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