Immune responses in mice and pigs after oral vaccination with rabies virus vectored Nipah disease vaccines

文献类型： 外文期刊

第一作者： Shuai, Lei

作者： Shuai, Lei;Ge, Jinying;Wen, Zhiyuan;Wang, Jinliang;Wang, Xijun;Bu, Zhigao;Bu, Zhigao

作者机构：

关键词： Rabies virus; Nipah virus; Attachment glycoprotein; Fusion glycoprotein; Oral vaccine

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：3.293； 五年影响因子：3.599 ）

ISSN： 0378-1135

年卷期： 2020 年 241 卷

页码：

收录情况： SCI

摘要： Nipah virus (NiV) is a re-emerging zoonotic pathogen that causes high mortality in humans and pigs. Oral immunization in free-roaming animals is one of the most practical approaches to prevent NiV pandemics. We previously generated a recombinant rabies viruses (RABV) Evelyn-Rokitnicki-Abelseth (ERA) strain, rERAG(333E), which contains a mutation from arginine to glutamic acid at residue 333 of glycoprotein (G(333E)) and serves as an oral vaccine for dog rabies. In this study, we generated two recombinant RABVs, rERAG(333E)/NiVG and rERAG(333E)/NiVF, expressing the NiV Malaysian strain attachment glycoprotein (NiV-G) or fusion glycoprotein (NiV-F) gene based on the rERAG 333E vector platform. Both rERAG(333E)/NiVG and rERAG(333E)/NiVF displayed growth properties similar to those of rERAG(333E) and caused marked syncytia formation after co-infection in BSR cell culture. Adult and suckling mice intracerebrally inoculated with the recombinant RABVs showed NiV-G and NW-F expression did not increase the virulence of rERAG(333E). Oral vaccination with rERAG(333E)/NiVG either singularly or combined with rERAG(333E)/NiVF induced significant NiV neutralizing antibody against NiV and RABV, and IgG to NiV-G or NiV-F in mice and pigs. rERAG(333E)/NiVG and rERAG(333E)/NiVF thus appeared to be suitable candidates for further oral vaccines for potential animal targets in endemic areas of NiV disease and rabies.

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