A sensitive lateral flow immunochromatographic strip with prussian blue nanoparticles mediated signal generation and cascade amplification

文献类型： 外文期刊

第一作者： Tian, Meiling

作者： Tian, Meiling;Xie, Wenyue;Zhang, Ting;Lu, Zhisong;Li, Chang Ming;Liu, Yingshuai;Liu, Yang

作者机构：

关键词： Immunochromatography; Signal amplification; Prussian blue nanoparticle; Colorimetric assay

期刊名称：SENSORS AND ACTUATORS B-CHEMICAL （ 影响因子：7.46； 五年影响因子：6.743 ）

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年卷期： 2020 年 309 卷

页码：

收录情况： SCI

摘要： This work reports a low-cost, fast and highly sensitive lateral flow immunoassay (LFIA) platform with Prussian blue nanoparticle (PBNP) as a new reporter, which efficiently mediates an on-demand cascade signal amplification by catalyzing oxidation of chromogenic reagent 3,3',5,5'-tetramethylbenzidine (TMB). In this case, PBNPs are accumulated on the test line through the specific immune interaction, resulting in a blue-colored line. The colorimetric signal can be further amplified via the peroxidase mimic PBNP-catalyzed TMB oxidation to produce an insoluble blue-violet precipitate. Under an optimal condition, a limit of detection (LOD) at 10 pg mL(-1) is achieved for rabbit IgG (RIgG) detection, which is 2-3 orders of magnitude better than the conventional LFIA strip using colloidal gold nanoparticle as a label. A macromolecular RIgG and a small molecular mycotoxin ochratoxin A (OTA) are also successfully analyzed in mimicking samples, demonstrating its good practicability of the proposed platform in a variety of fields.

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