G Protein-Gated Inwardly Rectifying Potassium Channel Subunit 3 is Upregulated in Rat DRGs and Spinal Cord After Peripheral Nerve Injury

文献类型： 外文期刊

第一作者： Lyu, Chuang

作者： Lyu, Chuang;Lyu, Gong-Wei;Mulder, Jan;Mulder, Jan;Martinez, Aurora;Shi, Tie-Jun Sten

作者机构：

关键词： GIRK channels; Kir3; axotomy; DRGs; spinal cord; chronic pain

期刊名称：JOURNAL OF PAIN RESEARCH （ 影响因子：3.133； 五年影响因子：3.507 ）

ISSN： 1178-7090

年卷期： 2020 年 13 卷

页码：

收录情况： SCI

摘要： Background: G protein-gated inwardly rectifying potassium (GIRK) channels are involved in the regulation of neuronal excitability. Four GIRK subunits (GIRK1-4) are expressed in rat dorsal root ganglia (DRGs). Recently, we have characterized the expression of GIRK1 and -2, and both are downregulated in rat DRGs and spinal cord after a complete sciatic nerve transection (axotomy). Here, we aimed to study the neurochemical characteristics of GIRK3, and its regulation in rat DRGs and spinal cord induced by nerve injury. Methods: A sciatic nerve axotomy was performed to study the influences of injury on GIRK3 expression in DRGs and spinal cord. A dorsal root rhizotomy and a sciatic nerve crush were employed to study the axonal transport of GIRK3 protein, respectively. Immunohistochemistry analysis was employed for investigating the neurochemical characteristics of GIRK3. Results: In control DRGs, similar to 18% of neuron profiles (NPs) were GIRK3-positive ((+)), and similar to 41%, similar to 48% and similar to 45% of GIRK3(+) NPs were CGRP(+), IB4(+) and NF200(+), respectively. GIRK3-like immunoreactivity was observed in glabrous skin of hind paws and axons originating from DRG neurons. Fourteen days after axotomy, more than one-third of DRG NPs were GIRK3(+), and among these similar to 51% and similar to 56% coexpressed galanin and neuropeptide Y, respectively. In control animals, a small group of interneurons found in the dorsal horn was GIRK3(+). In addition, GIRK3(+) processes could be observed in superficial laminae of spinal dorsal horn. After nerve injury, the intensity of GIRK3-like immunoreactivity in the superficial layers was increased. Evidence based on rhizotomy and sciatic nerve crush indicated both anterograde and retrograde transport of GIRK3. Conclusion: Our study demonstrates that GIRK3 is expressed in sensory neurons and spinal cord. GIRK3 has both anterograde and retrograde axonal transport. GIRK3 expression can be regulated by peripheral nerve injury.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序