Transcriptome and GWAS analyses reveal candidate gene for seminal root length of maize seedlings under drought stress

文献类型： 外文期刊

第一作者： Guo, Jian

作者： Guo, Jian;Yang, Deguang;Li, Chunhui;Li, Yongxiang;Zhang, Dengfeng;Shi, Yunsu;Song, Yanchun;Li, Yu;Wang, Tianyu;Zhang, Xiaoqiong

作者机构：

关键词： Maize; Drought stress; Seminal roots; RNA-seq; Genome-wide association study; Candidate gene

期刊名称：PLANT SCIENCE （ 影响因子：4.729； 五年影响因子：5.132 ）

ISSN： 0168-9452

年卷期： 2020 年 292 卷

页码：

收录情况： SCI

摘要： Water deficits are a major constraint on maize growth and yield, and deep roots are one of the major mechanisms of drought tolerance. In this study, four root and shoot traits were evaluated within an association panel consisting of 209 diverse maize accessions under well-watered (WW) and water-stressed (WS) conditions. A significant positive correlation was observed between seminal root length (SRL) under WS treatment and the drought tolerance index (DI) of maize seedlings. The transcriptome profiles of maize seminal roots were compared between four drought-tolerant lines and four drought-sensitive lines under both water conditions to identify genes associated with the drought stress response. After drought stress, 343 and 177 common differentially expressed genes (DEGs) were identified in the drought-tolerant group and drought-sensitive group, respectively. In parallel, a coexpression network underlying SRL was constructed on the basis of transcriptome data, and 10 hub genes involved in two significant associated modules were identified. Additionally, a genomewide association study (GWAS) of the SRL revealed 62 loci for the two water treatments. By integrating the results of the GWAS, the common DEGs and the coexpression network analysis, 7 promising candidate genes were prioritized for further research. Together, our results provide a foundation for the enhanced understanding of seminal root changes in response to drought stress in maize.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序