Development and utilization of an InDel marker linked to the fertility restorer genes of CMS-D8 and CMS-D2 in cotton

文献类型： 外文期刊

第一作者： Feng, Juanjuan

作者： Feng, Juanjuan;Wu, Jianyong;Zhu, Haiyong;Zhang, Meng;Zhang, Xuexian;Guo, Liping;Qi, Tingxiang;Tang, Huini;Wang, Hailin;Qiao, Xiuqin;Zhang, Bingbing;Shahzad, Kashif;Xing, Chaozhu

作者机构：

关键词： Cotton; Rf(2); Rf(1); InDel; CMS-D8; CMS-D2; MAS

期刊名称：MOLECULAR BIOLOGY REPORTS （ 影响因子：2.316； 五年影响因子：2.357 ）

ISSN： 0301-4851

年卷期： 2020 年 47 卷 2 期

页码：

收录情况： SCI

摘要： The cytoplasmic male sterility (CMS) system is a useful tool for commercial hybrid cotton seed production. Two main CMS systems, CMS-D8 and CMS-D2, have been recognized with Rf(2) and Rf(1) as the restorer genes, respectively. The development of molecular markers tightly linked with restorer genes can facilitate the breeding of restorer lines. In this study, the InDel-1892 marker was developed to distinguish Rf(2) and Rf(1) simultaneously. Sequence alignment implied that CMS-D8-Rf(2) has a 32 bp insertion and that CMS-D2-Rf(1) has a 186 bp insertion at the InDel-1892 locus. The codominant marker was co-segregated with Rf(1) and Rf(2). Hence, this marker can be used for tracing Rf(1) and Rf(2) simultaneously and identifying the allele status at the restorer gene locus. The results of this study will facilitate efficient marker-assisted selection for restorer lines and hybrids of CMS systems.

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