Development of a Tobacco Rootstock-Based Graft Inoculation Method for the Tomato Chlorosis Virus

文献类型： 外文期刊

第一作者： Dai, Huijie

作者： Dai, Huijie;Zhang, Ranran;Qiao, Ning;Liu, Jie;Li, Jingtang;Zhu, Xiaoping;Liu, Yongjie;Wang, Su

作者机构：

关键词： ToCV; Infectious clone; Graft inoculation; Bemisia tabaci; Nicotiana benthamiana

期刊名称：INTERNATIONAL JOURNAL OF AGRICULTURE AND BIOLOGY （ 影响因子：0.822； 五年影响因子：0.906 ）

ISSN： 1560-8530

年卷期： 2020 年 23 卷 4 期

页码：

收录情况： SCI

摘要： Tomato chlorosis virus (ToCV) spread by Bemisia tabaci has induced serious economic losses to the tomato industry around the world, and has aroused great attention from researchers. Due to the absence of a stable and efficient acquisition technology of ToCV, studies on the pathogenic mechanisms, interactions with hosts and the breeding of resistant varieties are greatly limited. In this study, we carried out relevant studies for the graft inoculation of ToCV on tobacco rootstock. An infectious cDNA clone of ToCV successfully infected Nicotiana benthamiana plants (the unnatural host), exhibiting typical symptoms, howevre, it could not make the tomato plants (the natural host) exhibit any symptom. Moreover, B. tabaci failed to infect by ToCV after feeding on the inoculated tomato plants. Grafting healthy tomato plants onto diseased N. benthamiana plants using different grafting methods all caused systemic infection of tomato plants. The incidence of ToCV in tomato plants grafted using cleft grafting was up to 79.08%, which was more suitable for the grafting of tomato plants onto N. benthamiana plants than approach grafting In addition, the virus from the grafted tomato plants could be transmitted to healthy tomato plants by the insect vector, B. tabaci. (C) 2020 Friends Science Publishers

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