Characterization of a novel glycoside hydrolase family 46 chitosanase, Csn-BAC, from Bacillus sp. MD-5

文献类型： 外文期刊

第一作者： Yang, Guosong

作者： Yang, Guosong;Mao, Xiangzhao;Sun, Huihui;Cao, Rong;Liu, Qi;Cao, Rong;Mao, Xiangzhao

作者机构：

关键词： Chitosanase; Chitosan; GH46 family

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：6.953； 五年影响因子：6.737 ）

ISSN： 0141-8130

年卷期： 2020 年 146 卷

页码：

收录情况： SCI

摘要： Chitosanases play an important role in chitosan degradation, and the enzymatic degradation products of chitosan show various biological activities. Here, a novel glycoside hydrolase family 46 chitosanase (named Csn-BAC) from Bacillus sp. MD-5 was heterologously expressed in Escherichia coli BL21 (DE3). The recombinant enzyme was purified by Ni-NTA affinity chromatography, and its molecular weight was estimated to be 35 kDa by SDS-PAGE. Csn-BAC showed maximal activity toward colloidal chitosan at pH 7 and 40 degrees C. The enzymatic activity of Csn-BAC was enhanced by Mn2+, Cu2+ and Co2+ at 1 mM, and by Mn2+ at 5 mM. Thin-layer chromatography and electrospray ionization-mass spectrometry results demonstrated that Csn-BAC exhibited an endo-type cleavage pattern and hydrolyzed chitosan to yield, mainly, (GlcN)(2) and (GlcN)(3). The enzymatic properties of this chitosanase may make it a good candidate for use in oligosaccharide production-based industries. (C) 2020 Elsevier B.V. All rights reserved.

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