Recombinant elongation factor 1 alpha of Haemonchus contortus affects the functions of goat PBMCs

文献类型： 外文期刊

第一作者： Ehsan, Muhammad

作者： Ehsan, Muhammad;Lu, MingMin;Yan, RuoFeng;Xu, LiXin;Song, XiaoKai;Li, XiangRui;Ehsan, Muhammad;Zhu, Xing-Quan;Gadahi, Javaid Ali;Du, AiFang;Hu, Min

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关键词： elongation factor-1 alpha; goat; Haemonchus contortus; peripheral blood mononuclear cells

期刊名称：PARASITE IMMUNOLOGY （ 影响因子：2.28； 五年影响因子：3.195 ）

ISSN： 0141-9838

年卷期： 2020 年 42 卷 5 期

页码：

收录情况： SCI

摘要： Excretory/secretory proteins of Haemonchus contortus (HcESPs) intermingle comprehensively with host immune cells and modulate host immune responses. In this study, H contortus ES antigen named as elongation factor 1 alpha (HcEF-1 alpha) was cloned and expressed. The influences of recombinant HcEF-1 alpha on multiple functions of goat peripheral blood mononuclear cells (PBMCs) were observed in vitro. Immunoblot analysis revealed that rHcEF-1 alpha was recognized by the serum of goat infected with H contortus. Immunofluorescence analysis indicated that rHcEF-1 alpha was bound on surface of PBMCs. Moreover, the productions of IL-4, TGF-beta 1, IFN-gamma and IL-17 of cells were significantly modulated by the incubation with rHcEF-1 alpha. The production of interleukin IL-10 was decreased. Cell migration, cell proliferation and cell apoptosis were significantly increased; however, nitric oxide production (NO) was significantly decreased. The MHC II molecule expression of cells incubated with rHcEF-1 alpha was increased significantly, whereas MHC-I was not changed as compared to the control groups (PBS control and pET32a). These findings indicated that rHcEF-1 alpha protein might play essential roles in functional regulations of HcESPs on goat PBMC and mediate the immune responses of the host during host-parasite relationship.

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