Biochemical Characterization and Mutational Analysis of a Lactone Hydrolase from Phialophora americana

文献类型： 外文期刊

第一作者： Yu, Xinrui

作者： Yu, Xinrui;Tu, Tao;Luo, Huiying;Huang, Huoqing;Su, Xiaoyun;Wang, Yuan;Wang, Yaru;Zhang, Jie;Bai, Yingguo;Yao, Bin

作者机构：

关键词： zearalenone; lactone hydrolase; Phialophora americana; site-directed mutagenesis; molecular dynamics

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：5.279； 五年影响因子：5.269 ）

ISSN： 0021-8561

年卷期： 2020 年 68 卷 8 期

页码：

收录情况： SCI

摘要： The mycotoxin zearalenone (ZEN) is a secondary metabolite produced mainly by Fusarium species. ZEN poses health hazards both for humans and animals, as a major contaminant in the food and feed industries. Currently, there is no effective technique for degrading ZEN during industrial processes. In this study, we isolated and biochemically characterized a novel lactone hydrolase, ZHD607, isolated from Phialophora americana, cloned, and exogenously expressed in Pichia pastoris. ZHD607 was characterized as a mesophilic lactone hydrolase having a neutral pH and showing optimal activity at 35 degrees C and pH 8.0. Two mutants, ZHDM1 and 1160Y, generated from ZHD607 based on structure and sequence alignment analyses, exhibited 2.9- and 3.4-fold higher activity towards ZEN than did ZHD607. Molecular dynamics simulation revealed diverse mechanisms driving this improved catalytic activity. These findings enrich our knowledge about ZHD enzyme family and represent an important step toward industrialization of ZEN-detoxifying lactone hydrolases.

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